Ikbkg CLIA Kit for Mouse(Mus musculus) | EIAab.comIkbkg CLIA Kit for Mouse(Mus musculus)

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Ikbkg (Gene name),

NF-kappa-B essential modulator (Protein name ), NEMO_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Ikbkg(Nemo);

Protein name:

NF-kappa-B essential modulator(NEMO);

Alternative:

Inhibitor of nuclear factor kappa-B kinase subunit gamma(I-kappa-B kinase subunit gamma;IKK-gamma;IKKG;IkB kinase subunit gamma);IkB kinase-associated protein 1(IKKAP1;mFIP-3);NF-kappa-B essential modifier;

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Homodimer; disulfide-linked. Component of the I-kappa-B-kinase (IKK) core complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits. The IKK core complex seems to associate with regulatory or adapter proteins to form a IKK-signalosome holo-complex. The IKK complex associates with TERF2IP/RAP1, leading to promote IKK-mediated phosphorylation of RELA/p65. Part of a complex composed of NCOA2, NCOA3, CHUK/IKKA, IKBKB, IKBKG and CREBBP. Interacts with COPS3, CYLD, NALP2, TRPC4AP and LRDD. Interacts with ATM; the complex is exported from the nucleus. Interacts with TRAF6. Interacts with TANK; the interaction is enhanced by TBK1 and IKBKE. Part of a ternary complex consisting of TANK, IKBKB and IKBKG. Interacts with ZFAND5. Interacts with RIPK2.

Function:

Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity.

Subcellular Location:

Cytoplasm Nucleus

Protein Attributes:

Sequence length:

412

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

412:

DTLQIHVMEC | IE

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

PF11577

STRING:

10090.ENSMUSP00000004330

KEGG:

mmu:16151

UniGene:

Mm.12967

String:

O88522

SMR:

O88522

Uniprot:

O88522

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ELISA Kit for Mouse NEMO

Cat.:

E9868h

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96T

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ELISA Kit for Mouse NEMO

Cat.:

E9868m

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ELISA Kit for Mouse NEMO

Cat.:

E9868r

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96T

ELISA Kit for Mouse NEMO

Cat.:

E9868b

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96T

CLIA Kit for Mouse NEMO

Cat.:

U9868m

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96T

CLIA Kit for Mouse NEMO

Cat.:

U9868b

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96T

CLIA Kit for Mouse NEMO

Cat.:

U9868r

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96T

CLIA Kit for Mouse NEMO

Cat.:

U9868h

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Polyclonal Antibody for Mouse NEMO

Cat.:

P9868Rb-m

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40ug/0.2ml

Polyclonal Antibody for Mouse NEMO

Cat.:

P9868Rb-h

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40ug/0.2ml

Monoclonal Antibody for Mouse NEMO

Protein for Mouse NEMO

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.2%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of NEMOinto serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	89-102
Plasma	97	91-105

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NEMO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-103%	92-102%	89-104%	91-107%
EDTA plasma(n=5)	93-101%	94-100%	90-103%	89-106%
heparin plasma(n=5)	92-103%	91-105%	94-101%	93-102%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Complementation cloning of NEMO, a component of the I-kappaB kinase complex essential for NF-kappaB activation."
Yamaoka S. , Courtois G. , Bessia C. , Whiteside S.T. , Weil R. , Agou F. , Kirk H.E. , Kay R.J. , Israel A.
Cell93:1231-1240(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: T-cell.
2.

"Human-mouse comparative sequence analysis of the NEMO gene reveals an alternative promoter within the neighboring G6PD gene."
Galgoczy P. , Rosenthal A. , Platzer M.
Gene271:93-98(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
3.

"Ikbkg gene modulates the herpes virus susceptibility in mice."
Perelygin A.A. , Perelygina L.M.
Submitted (2002-05) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: BALB/c.
tissue: Liver.
4.

"The transcriptional landscape of the mammalian genome."
Carninci P. , Kasukawa T. , Katayama S. , Gough J. , Frith M.C. , Maeda N. , Oyama R. , Ravasi T. , Lenhard B. , Wells C. , Kodzius R. , Shimokawa K. , Bajic V.B. , Brenner S.E. , Batalov S. , Forrest A.R. , Zavolan M. , Davis M.J. , more...
Science309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
strain: NOD.
5.

"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M. , Goodstadt L. , Hillier L.W. , Zody M.C. , Goldstein S. , She X. , Bult C.J. , Agarwala R. , Cherry J.L. , DiCuccio M. , Hlavina W. , Kapustin Y. , Meric P. , Maglott D. , Birtle Z. , Marques A.C. , Graves T. , Zhou S. , more...
PLoS Biol.7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
strain: C57BL/6J.
6.

Mural R.J. , Adams M.D. , Myers E.W. , Smith H.O. , Venter J.C.
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
7.

"IkappaB kinase (IKK)-associated protein 1, a common component of the heterogeneous IKK complex."
Mercurio F. , Murray B.W. , Shevchenko A. , Bennett B.L. , Young D.B. , Li J.W. , Pascual G. , Motiwala A. , Zhu H. , Mann M. , Manning A.M.
Mol. Cell. Biol.19:1526-1538(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 85-412;PROTEIN SEQUENCE OF 144-159
tissue: Cervix carcinoma.
8.

"Identification of a cell protein (FIP-3) as a modulator of NF-kappaB activity and as a target of an adenovirus inhibitor of tumor necrosis factor alpha-induced apoptosis."
Li Y. , Kang J. , Friedman J. , Tarassishin L. , Ye J. , Kovalenko A. , Wallach D. , Horwitz M.S.
Proc. Natl. Acad. Sci. U.S.A.96:1042-1047(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
9.

"Role of ikkgamma/nemo in assembly of the IkappaB kinase complex."
Li X.-H. , Fang X. , Gaynor R.B.
J. Biol. Chem.276:4494-4500(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IKK COMPLEX
10.

"Regulation of Ikappa B kinase (IKK)gamma /NEMO function by IKKbeta -mediated phosphorylation."
Prajapati S. , Gaynor R.B.
J. Biol. Chem.277:24331-24339(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-369;MUTAGENESIS OF SER-369 AND SER-375
11.

"Association of the adaptor TANK with the I kappa B kinase (IKK) regulator NEMO connects IKK complexes with IKK epsilon and TBK1 kinases."
Chariot A. , Leonardi A. , Muller J. , Bonif M. , Brown K. , Siebenlist U.
J. Biol. Chem.277:37029-37036(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TANK AND IKBKB
12.

"ABIN-1 binds to NEMO/IKKgamma and co-operates with A20 in inhibiting NF-kappaB."
Mauro C. , Pacifico F. , Lavorgna A. , Mellone S. , Iannetti A. , Acquaviva R. , Formisano S. , Vito P. , Leonardi A.
J. Biol. Chem.281:18482-18488(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TNIP1 AND TNFAIP3
13.

"Identification of TRAF6-dependent NEMO polyubiquitination sites through analysis of a new NEMO mutation causing incontinentia pigmenti."
Sebban-Benin H. , Pescatore A. , Fusco F. , Pascuale V. , Gautheron J. , Yamaoka S. , Moncla A. , Ursini M.V. , Courtois G.
Hum. Mol. Genet.16:2805-2815(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION AT LYS-278; LYS-314; LYS-318; LYS-319 AND LYS-392;MUTAGENESIS OF LYS-278; LYS-314; VAL-316; LYS-318; LYS-319 AND LYS-392
14.

"Telomere-independent Rap1 is an IKK adaptor and regulates NF-kappaB-dependent gene expression."
Teo H. , Ghosh S. , Luesch H. , Ghosh A. , Wong E.T. , Malik N. , Orth A. , de Jesus P. , Perry A.S. , Oliver J.D. , Tran N.L. , Speiser L.J. , Wong M. , Saez E. , Schultz P. , Chanda S.K. , Verma I.M. , Tergaonkar V.
Nat. Cell Biol.12:758-767(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TERF2IP
15.

"Specific recognition of linear ubiquitin chains by NEMO is important for NF-kappaB activation."
Rahighi S. , Ikeda F. , Kawasaki M. , Akutsu M. , Suzuki N. , Kato R. , Kensche T. , Uejima T. , Bloor S. , Komander D. , Randow F. , Wakatsuki S. , Dikic I.
Cell136:1098-1109(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 250-343 IN COMPLEX WITH UBIQUITIN;UBIQUITIN-BINDING;MUTAGENESIS OF VAL-293; TYR-301; LYS-302; PHE-305; ARG-309; ARG-312; GLU-313; GLU-313; GLU-317 AND GLU-320
16.

"DARPin-assisted crystallography of the CC2-LZ domain of NEMO reveals a coupling between dimerization and ubiquitin binding."
Grubisha O. , Kaminska M. , Duquerroy S. , Fontan E. , Cordier F. , Haouz A. , Raynal B. , Chiaravalli J. , Delepierre M. , Israel A. , Veron M. , Agou F.
J. Mol. Biol.395:89-104(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 251-337;UBIQUITIN-BINDING;OLIGOMETRIZATION

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